Nucleotide exchange factors
نویسندگان
چکیده
منابع مشابه
Nucleotide exchange factors
Exchange factors are enzymes that catalyze the exchange of GTP for GDP on guanine nucleotide binding proteins. Progress in understanding the molecular basis of action and the cellular functions of these enzymes has largely come from structural determinations (e.g., crystal structures) and studying effects on cells when expression levels of the exchange factors are perturbed or mutated exchange ...
متن کاملQuantitative Analysis of Guanine Nucleotide Exchange Factors (GEFs) as Enzymes
The proteins that possess guanine nucleotide exchange factor (GEF) activity, which include about ~800 G protein coupled receptors (GPCRs),1 15 Arf GEFs,2 81 Rho GEFs,3 8 Ras GEFs,4 and others for other families of GTPases,5 catalyze the exchange of GTP for GDP on all regulatory guanine nucleotide binding proteins. Despite their importance as catalysts, relatively few exchange factors (we are aw...
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Molecular chaperones of the Hsp70 family form an important hub in the cellular protein folding networks in bacteria and eukaryotes, connecting translation with the downstream machineries of protein folding and degradation. The Hsp70 folding cycle is driven by two types of cochaperones: J-domain proteins stimulate ATP hydrolysis by Hsp70, while nucleotide exchange factors (NEFs) promote replacem...
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We have previously described a fluorimetric method to measure ADP-ATP exchange rates in mitochondria of permeabilized cells where phosphorylases, kinases, ion pumps exhibiting substantial ATP hydrolase activity and other competing reactions interconverting adenine nucleotides are present. This was achieved by recording changes in free extramitochondrial [Mg] reported by the Mg-sensitive fluores...
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ژورنال
عنوان ژورنال: Cellular Logistics
سال: 2012
ISSN: 2159-2799
DOI: 10.4161/cl.21627